Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters
Само за регистроване кориснике
2021
Аутори
Kolarević, StoimirMicsinai, Adrienn
Szántó-Egész, Réka
Lukács, Alena
Kračun-Kolarević, Margareta
Lundy, Lian
K.T. Kirschner, Alexander
H. Farnleitner, Andreas
Djukic, Aleksandar
Čolić, Jasna
Nenin, Tanja
Sunjog, Karolina
Paunović, Momir
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was det...ected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
Кључне речи:
SARS-CoV-2 / Danube River / Faecal pollution / Untreated wastewaters / SerbiaИзвор:
Science of the Total Environment, 2021, 783Колекције
Институција/група
GraFarTY - JOUR AU - Kolarević, Stoimir AU - Micsinai, Adrienn AU - Szántó-Egész, Réka AU - Lukács, Alena AU - Kračun-Kolarević, Margareta AU - Lundy, Lian AU - K.T. Kirschner, Alexander AU - H. Farnleitner, Andreas AU - Djukic, Aleksandar AU - Čolić, Jasna AU - Nenin, Tanja AU - Sunjog, Karolina AU - Paunović, Momir PY - 2021 UR - https://grafar.grf.bg.ac.rs/handle/123456789/2352 AB - In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment. T2 - Science of the Total Environment T1 - Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters VL - 783 DO - 10.1016/j.scitotenv.2021.146967 ER -
@article{ author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Lundy, Lian and K.T. Kirschner, Alexander and H. Farnleitner, Andreas and Djukic, Aleksandar and Čolić, Jasna and Nenin, Tanja and Sunjog, Karolina and Paunović, Momir", year = "2021", abstract = "In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.", journal = "Science of the Total Environment", title = "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters", volume = "783", doi = "10.1016/j.scitotenv.2021.146967" }
Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Lundy, L., K.T. Kirschner, A., H. Farnleitner, A., Djukic, A., Čolić, J., Nenin, T., Sunjog, K.,& Paunović, M.. (2021). Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment, 783. https://doi.org/10.1016/j.scitotenv.2021.146967
Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Lundy L, K.T. Kirschner A, H. Farnleitner A, Djukic A, Čolić J, Nenin T, Sunjog K, Paunović M. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment. 2021;783. doi:10.1016/j.scitotenv.2021.146967 .
Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Lundy, Lian, K.T. Kirschner, Alexander, H. Farnleitner, Andreas, Djukic, Aleksandar, Čolić, Jasna, Nenin, Tanja, Sunjog, Karolina, Paunović, Momir, "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters" in Science of the Total Environment, 783 (2021), https://doi.org/10.1016/j.scitotenv.2021.146967 . .