GraFar - Repository of the Faculty of Civil Engineering
Faculty of Civil Engineering of the University of Belgrade
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   GraFar
  • GraFar
  • Катедра за хидротехнику и водно-еколошко инжењерство
  • View Item
  •   GraFar
  • GraFar
  • Катедра за хидротехнику и водно-еколошко инжењерство
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters

Authorized Users Only
2021
Authors
Kolarević, Stoimir
Micsinai, Adrienn
Szántó-Egész, Réka
Lukács, Alena
Kračun-Kolarević, Margareta
Lundy, Lian
K.T. Kirschner, Alexander
H. Farnleitner, Andreas
Djukic, Aleksandar
Čolić, Jasna
Nenin, Tanja
Sunjog, Karolina
Paunović, Momir
Article (Published version)
,
the Authors
Metadata
Show full item record
Abstract
In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was det...ected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.

Keywords:
SARS-CoV-2 / Danube River / Faecal pollution / Untreated wastewaters / Serbia
Source:
Science of the Total Environment, 2021, 783

DOI: 10.1016/j.scitotenv.2021.146967

ISSN: 0048-9697

WoS: 000656998000014

[ Google Scholar ]
8
URI
https://grafar.grf.bg.ac.rs/handle/123456789/2352
Collections
  • Катедра за хидротехнику и водно-еколошко инжењерство
  • Radovi istraživača / Researcher's publications
Institution/Community
GraFar
TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Micsinai, Adrienn
AU  - Szántó-Egész, Réka
AU  - Lukács, Alena
AU  - Kračun-Kolarević, Margareta
AU  - Lundy, Lian
AU  - K.T. Kirschner, Alexander
AU  - H. Farnleitner, Andreas
AU  - Djukic, Aleksandar
AU  - Čolić, Jasna
AU  - Nenin, Tanja
AU  - Sunjog, Karolina
AU  - Paunović, Momir
PY  - 2021
UR  - https://grafar.grf.bg.ac.rs/handle/123456789/2352
AB  - In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.
T2  - Science of the Total Environment
T1  - Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters
VL  - 783
DO  - 10.1016/j.scitotenv.2021.146967
ER  - 
@article{
author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Lundy, Lian and K.T. Kirschner, Alexander and H. Farnleitner, Andreas and Djukic, Aleksandar and Čolić, Jasna and Nenin, Tanja and Sunjog, Karolina and Paunović, Momir",
year = "2021",
abstract = "In Serbia less than 13% of collected municipal wastewaters is being treated before their release in the environment. This includes all municipal wastewater discharges from Belgrade (capital city of Serbia; population 1,700,000). Previous research has identified the impacts of raw wastewater discharges from Belgrade on the Danube River, and this study investigated if such discharges also provided a pathway for SARS-CoV-2 RNA material. Samples were collected during the most critical circumstances that occurred so far within the COVID-19 pandemics in Serbia. Grab and composite samples were collected in December 2020, during the peak of the third wave (in terms of reported cases) at the site which receives the wastewater loads in Belgrade. Grab samples collected upstream and downstream of Belgrade were also analyzed. RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. SARS-CoV-2 RNA (5.97 × 103 to 1.32 × 104 copies/L) was detected only in samples collected at the site strongly impacted by the wastewaters where all three applied primer sets gave positive signals. Determined concentrations correspond to those reported in wastewater influents sampled at treatment plants in other countries indicating an epidemiological indicator function of used approach for rivers with high pollution loads in countries with poor wastewater treatment.",
journal = "Science of the Total Environment",
title = "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters",
volume = "783",
doi = "10.1016/j.scitotenv.2021.146967"
}
Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Lundy, L., K.T. Kirschner, A., H. Farnleitner, A., Djukic, A., Čolić, J., Nenin, T., Sunjog, K.,& Paunović, M.. (2021). Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment, 783.
https://doi.org/10.1016/j.scitotenv.2021.146967
Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Lundy L, K.T. Kirschner A, H. Farnleitner A, Djukic A, Čolić J, Nenin T, Sunjog K, Paunović M. Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters. in Science of the Total Environment. 2021;783.
doi:10.1016/j.scitotenv.2021.146967 .
Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Lundy, Lian, K.T. Kirschner, Alexander, H. Farnleitner, Andreas, Djukic, Aleksandar, Čolić, Jasna, Nenin, Tanja, Sunjog, Karolina, Paunović, Momir, "Detection of SARS-CoV-2 RNA in the Danube River in Serbia associated with the discharge of untreated wastewaters" in Science of the Total Environment, 783 (2021),
https://doi.org/10.1016/j.scitotenv.2021.146967 . .

DSpace software copyright © 2002-2015  DuraSpace
About the GraFar Repository | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About the GraFar Repository | Send Feedback

OpenAIRERCUB